Table 1.
Mutational status of the edited genotypes, as revealed by the TIDE analysis of the Sanger sequences. Four regions surrounding the adopted sgRNA were analyzed.
| Mutant | Mutational Status (%)-TIDE | |||
|---|---|---|---|---|
| sgRNA6 | sgRNA8 | sgRNA1 | sgRNA7 | |
| SM1 | 1.2 | - | - | - |
| SM2 | 2.5 | - | - | - |
| SM3 | 8.7 | - | - | - |
| SM4 | 38.5 | 89.4 | - | 98 |
| SM5 | 24.2 | - | - | 97.6 |
| SM6 | 40.2 | 95.1 | 4.1 | 98.9 |
| SM7 | 13.1 | 94.5 | 58.1 | - |
| SM8 | 34.6 | 95.5 | 33.6 | - |
| SM9 | - | 95.4 | 35.3 | - |
| SM12 | - | 93.3 | 9.1 | - |
| SM13 | 93.4 | 90.3 | 48.1 | 99 |
| SM14 | - | 92.8 | 3.4 | - |
| SM16 | - | 97.8 | 7.6 | - |
| SM17 | 64.6 | 94.2 | 76.2 | 97.9 |
| SM18 | - | 92.9 | 80.1 | - |
| SM19 | 1.5 | 93.1 | 31.3 | 99.3 |
| SM20 | 1.8 | 1.1 | 3.7 | 2.2 |
| SM22 | 69.3 | 94.2 | 9.7 | 98.3 |
| SM24 | - | 93.6 | 34.2 | - |
| SM25 | - | 94.2 | 5.2 | - |
| SM26 | - | 93.9 | 4.4 | - |
| OX1 | 55.3 | - | - | - |
| OX2 | 17.7 | 98.6 | 6.6 | 99 |
| OX3 | 26.8 | 98.2 | 56.7 | 99.3 |
| OX4 | 14.2 | 99 | 9.9 | 99 |
| OX9 | 13.5 | 95.9 | 4.7 | 99.3 |
| OX11 | 48.6 | 94.4 | 32.5 | 99.3 |