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. 2022 Nov 22;14(23):4943. doi: 10.3390/nu14234943

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of combined genistein and daidzein on EFS- and agonist-induced contractions of human prostate tissues. Contractions were induced by EFS (A), noradrenaline (B), phenylephrine (C), methoxamine (D), endothelin-1 (E) and U46619 (F) in the presence of 6 µM genistein plus 5 µM daidzein and corresponding amounts of solvent (DMSO) for the controls added 30 min before the construction of frequency and concentration–response curves. Data from experiments with tissues from n = 5 patients are shown in each diagram. In each experiment, tissue from one patient was allocated to the control and genistein + daidzein groups. Data are means ± SDs in frequency/concentration–response curves (p-values for whole groups from two-way ANOVA), and E_max, Ef₅₀ and EC₅₀ values are from all single experiments (calculated by curve fitting) in scatter plots (p-values from two-tailed t-tests).