Figure 9.

Effects of genistein, daidzein and puerarin on apoptosis and cell death in WPMY-1 cells. Numbers of cells in apoptosis (annexin V-positive, 7-AAD-negative) and of dead cells (resulting from apoptosis and/or necrosis; annexin V-positive, 7-AAD-positive) were assessed by flow cytometry. Flow cytometry was performed following culture with genistein (A), daidzein (B) and puerarin (C) at the indicated concentrations or solvent (DMSO for genistein and daidzein, methanol for puerarin) for the controls for 24 h. Shown are all single values together with means from the quantification of five independent experiments and representative experiments for each series, along with p-values from one-way ANOVA with Dunnett’s tests.