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Coomassie blue-stained gel (A) and Western immunoblot using specific anti-porin II serum (B) of OM proteins from different bacterial strains. Lanes: 1, A. hydrophila AH-3 (wild-type, serogroup O:34); 2, size standard (20, 31, 43, 62, and 97 kDa); 3, E. coli DHα5 harboring cosmid pLA2917; 4, E. coli DH5α harboring pLA-POR3 plasmid DNA (carrying the porin II gene); 5, the same strain as in lane 4 lacking the pLA-POR3 plasmid DNA. The arrow points to porin II (39 kDa).
