TABLE 1.
Bacterial strains, cosmids, and plasmids used
| Strain, cosmid, or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | F−endA hsdR17 (rk− mk+) supE44 thi-1 recA1 gyr-A96 φ80lacZ | 9 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F′ proAB lacIZΔM15 Tn10) | Stratagene |
| MC1061λpir | thi thr 1 leu6 proA2 his4 argE2 lacY1 galK2 ara14 xyl5 supE44 λpir | 27 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr λpir | 27 |
| Aeromonas sp. | ||
| AH-3 | A. hydrophila wild type, serogroup O:34 | 20 |
| AH-405 | Rifampin-resistant mutant derived from AH-3 | 23 |
| AH-53 | Serum-sensitive rough mutant derived from AH-3 | 24 |
| AH-330 | Porin II insertion AH-405 mutant obtained with pFS-POR3 | This work |
| AH-336 | Porin II insertion AH-53 mutant obtained with pFS-POR3 | This work |
| AH-334 | Mutant AH-330 complemented by plasmid pLA-POR6 | This work |
| AH-338 | Mutant AH-336 complemented by plasmid pLA-POR6 | This work |
| TF7 | A. veronii sobria wild type, serogroup O:11 | 24 |
| AH-408 | Rifampin-resistant mutant derived from TF7 | This work |
| AH-26 | Rough S− serum-sensitive mutant derived from TF7 | 24 |
| AH-331 | Porin II insertion AH-408 mutant obtained with pFS-POR3 | This work |
| AH-337 | Porin II insertion AH-26 mutant obtained with pFS-POR3 | This work |
| AH-335 | Mutant AH-331 complemented by plasmid pLA-POR6 | This work |
| AH-339 | Mutant AH-337 complemented by plasmid pLA-POR6 | This work |
| Plasmids | ||
| pLA2917 | Tcr Kmr | 3 |
| COS-POR2 | pLA2917 with 20-kb chromosomal AH-3 Sau3A insert (porin II gene) | This work |
| pLA-POR3 | pLA2917 with 7.8-kb BglII insert from DNA insert of COS-POR2 (porin II gene) | This work |
| pLA-POR6 | pLA2917 with the single porin II gene | This work |
| pFS100 | pGP704 suicide plasmid, λpir-dependent, Kmr | 27 |
| pFS-POR3 | pFS100 with an internal fragment (996 bp) of porin II gene | This work |