TABLE 3.
Serum susceptibility and binding of 125I-labeled C1q to mesophilic Aeromonas sp. whole cells
| Strain | Relevant characteristic(s) | % Survival after 3 h in NHSa | Bound C1q (molecules/bacterial cell)a |
|---|---|---|---|
| AH-3 | O:34 (smooth)b | 101 ± 4 | 89 ± 17 |
| AH-3 | O:34 (rough)c | <0.1 | 304 ± 37 |
| AH-405 | AH-3 Rifr (smooth) | 103 ± 3 | 87 ± 18 |
| AH-405 | AH-3 Rifr (rough) | <0.1 | 309 ± 40 |
| AH-53 | O− derived from AH-3 (20) | <0.1 | 378 ± 42 |
| AH-330 | Porin II mutant of AH-405 (smooth) | 108 ± 6 | <30 |
| AH-330 | Porin II mutant of AH-405 (rough) | 105 ± 4 | <30 |
| AH-334 | AH-330 + pLA-POR6 (smooth) | 99 ± 3 | 89 ± 15 |
| AH-334 | AH-330 + pLA-POR6 (rough) | <0.1 | 317 ± 33 |
| AH-336 | Porin II mutant of AH-53 | 98 ± 5 | <30 |
| AH-338 | AH-336 + pLA-POR6 | <0.1 | 369 ± 36 |
| TF7 | O:11, S+ | 145 ± 12 | <10 |
| AH-408 | TF7 Rifr | 139 ± 8 | <10 |
| AH-26 | O− S−, derived from TF7 (22) | <0.1 | 389 ± 38 |
| AH-331 | Porin II mutant of AH-408 | 141 ± 6 | <10 |
| AH-335 | AH-331 + pLA-POR6 | 143 ± 7 | <10 |
| AH-337 | Porin II mutant of AH-26 | 129 ± 8 | <30 |
| AH-339 | AH-337 + pLA-POR6 | <0.1 | 376 ± 35 |
a
Average of at least three independent experiments ± standard deviation. Some of the data have been previously published (24).
b
Strains of serogroup O:34 showed O:34 antigen LPS (smooth) when grown at 20°C (20).
c
Strains of serogroup O:34 are unable to show O:34 antigen LPS (rough) when grown at 37°C and in low-osmolarity medium (1).