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. 2022 Dec 6;14(23):6006. doi: 10.3390/cancers14236006

Figure 4.

Figure 4

Global gene expression analysis revealed activation of the BCR-mediated signalling pathway in PKCα-KR cells. Global RNA analysis was performed using Affymetrix GeneChip mouse gene 1.0 ST on MIEV and PKCα-KR transduced cells at d17–23 in the B cell transformation co-culture. (A) Dysregulated components of the BCR pathway in PKCα-KR vs. MIEV cells isolated from late co-cultures (n = 5 PKCα-KR, n = 5 MIEV) were identified. Significantly up- and down-regulated components are highlighted in pink/red and green, respectively; (B) qPCR validation of egr1 and btk genes, which were both upregulated in the microarray analysis at the late stage (d15–23) of B cell transformation, compared with the early stage (d6–10). Data are an average of n = 2–4 biological replicates, normalised to tbp. (C) Comparison of prkcb, sp1 and lef1 gene expression in the early vs. late stages of B cell transformation co-cultures, determined by qPCR. Data represent n = 3–5 biological replicates, normalised to gapdh. Unpaired student t-tests were used to analyse the data. * p < 0.05, ** p < 0.01.