Table 1.
Donor details. Samples 1–7 originate from the eye bank; these specimens have been maintained in culture; samples 5b–7b were used to assess the effect of FFPE embedding; samples 8–10 are corneas fixed immediately after enucleation and were not stored in tissue culture medium (TC). Necrosis (necrotic endothelial cells in percent compared to live endothelial cells). M: male, F: female, NA: not applicable.
| Donor | Age at Death [years] |
Sex | Endothelial Cell Count [cells/mm²] |
Necrosis [%] |
Time between Death and Corneal Extraction [days] |
Time in Culture [days] |
Specifics | Tissue Culture (TC) | Corneal Graft Diameter |
|---|---|---|---|---|---|---|---|---|---|
| Corneal tissue in culture unfixed samples | |||||||||
| 1 | 60 | M | 1898 | 20 | 1 | 42 | / | M2 | 9.0 mm |
| 2 | 59 | M | 949 | 10 | 1 | 36 | Pseudophakic | M2 | 9.0 mm |
| 3 | 87 | F | 1241 | 5 | 2 | 35 | Corneal scar | M2 | 9.0 mm |
| 4 | 82 | M | 803 | 50 | 0 | 21 | / | M2 | 9.0 mm |
| 5a | 88 | M | 1752 | 3 | 1 | 57 | Pseudophakic, Arcus lipoides | M2 | ½ 9.0 mm |
| 6a | 78 | M | 1825 | 3 | 1 | 56 | Arcus lipoides | M2 | ½ 9.0 mm |
| 7a | 87 | M | 2117 | 10 | 1 | 40 | Arcus lipoides | M2 | ½ 9.0 mm |
| Corneal tissue in culture FFPE fixed samples | |||||||||
| 5b | As in 5a | ½ 9.0 mm | |||||||
| 6b | As in 6a | ½ 9.0 mm | |||||||
| 7b | As in 7a | ½ 9.0 mm | |||||||
| Healthy corneal samples (FFPE fixed) | |||||||||
| 8 | 55 | M | Not measured | Not measured | Fixed directly after enucleation | NA | Uveal melanoma | No TC contact | 9.0 mm |
| 9 | 81 | M | Not measured | Not measured | Fixed directly after enucleation | NA | Uveal melanoma | No TC contact | 9.0 mm |
| 10 | 73 | F | Not measured | Not measured | Fixed directly after enucleation | NA | Uveal melanoma | No TC contact | 9.0 mm |