Table 1.

Mini-reporter genes for Tau exons splicing evaluation.

Minigene Name	Exon(s)	Properties	Application	Ref.
hTau	1, 2, 4	Exon 2 flanked by different length of its native introns, surrounded by E1 and E4	Identification of cis and trans acting elements altering splicing of E2	[132]
SV1/2L/4	2	Exon 2 flanked by its native exons	In situ mutagenesis to identify cis acting elements in the exon and downstream intron	[36]
SP/2L	2	Exon 2 flanked by heterologous exons	In situ mutagenesis to identify cis acting elements in the exon and downstream intron	[36]
SVΔ2/3	3	E3, 1.1 kbps of I2 and 1.4 kpbs of I3	E3 default splicing pattern analysis, identification of cis-acting elements	[37]
pSVIRB SV6	6	E6, 600 bps I5, 200 bps I6	Exon-trapping assay	[40]
TauEx9-11d5	9-10-11	E10 flanked by 269 and 246 bp of its native intron and exons 9–11.	Identification of cis-acting elements altering splicing of E10	[129]
SI9/SI10 LI9/SI10 SI9/LI10 LI9/LI10	9-10-11	E10 can be flanked by a long (L) or short (S) portion of the upstream and downstream intron	Identification of a required minimal distance for E10 splicing	[130]
Tau minigene	9-10-11		Identification of HnRNPC as a novel trans-acting factor on E10	[133]
RHCGlo Tau wt RHCGlo Tau Δ280K RHCGlo Tau N279K	10	Contains WT E10 or E10 with Δ280K, and N279K mutations	Identification of cis and trans-acting elements affecting E10 splicing	[128]
Luc M14	10	Luciferase reporter, modified from TauEx9-11 d5 minigene	Identification of compound that reduced exon 10 inclusion; identify cis/trans elements	[130,131,134,135]