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. 2022 Nov 30;11(23):3849. doi: 10.3390/cells11233849

Figure 5.

Figure 5

Transcriptomic and proteomic analysis of the spleen at 7 d after multifrequency microwave exposure. At 7 d after microwave exposure, the rats were euthanized, and the spleens were removed from the Sham, S10, X10 and XS10 groups. (A–C) Transcriptomics analysis. Total RNA was extracted by TRIzol reagent and RNA sequencing was performed using Illumina NovaSeq, and the DEGs were analyzed as described above (A). The mRNA expression levels of four DEGs were verified by qRT-PCR (B), and four other immune activity-associated genes were also measured by qRT-PCR (C). (D,E) Proteomics analysis. The proteins were extracted from the spleens. After the spectral library was constructed, the DEPs were analyzed by DDA mode (D). The protein expression of four DEPs was verified by mass spectrometry (E). Data are shown as mean±s.e.m.; *, p < 0.05, **, p < 0.01, ***, p < 0.001 vs. Sham group; #, p < 0.05, ##, p < 0.01, ###, p < 0.001 vs. S10 group; $, p < 0.05, $$, p < 0.01 vs. X10 group.