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. 2022 Dec 2;11(23):3351. doi: 10.3390/plants11233351

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Local and systemic symptoms induced by Potato virus X (PVX) expression of V2 from beet curly top Iran virus (PVX-IVV2) in wt Nicotiana benthamiana plants. Empty PVX vector (PVX) was used as negative control. Recombinant PVX viruses expressing either the nonstructural protein (NSs) from tomato spotted wilt virus (PVX-NSs) or the V2 protein from beet curly top virus (PVX-BCV2) were used as positive controls. (A) Local symptoms induced in the infiltrated area at 6 dpi. (B) Representative plants showing systemic symptoms at 16 dpi. Four to six plants were agroinfiltrated per experiment. Similar results were obtained in three independent experiments.