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. 2022 Dec 2;11(23):3351. doi: 10.3390/plants11233351

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Subcellular localization of V2 from beet curly top Iran virus fused to GFP in epidermal cells of wild type Nicotiana benthamiana. (A) Leaves were agroinfiltrated with a construct expressing the GFP (GFP), GFP-V2 fusion protein from beet curly top Iran virus (GFP-IVV2), or the GFP-V2 fusion protein from beet curly top virus (GFP-BCV2). Samples were observed under the confocal microscope at 36 h post infection. (B) Close up confocal images of the observed areas. GFP fluorescence (GFP), autofluorescence (AF), and the bright field channel (BF) are shown.