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Confirmation of construction of allelic replacement mutants in the opp locus. (A) Southern blots of ScaI-digested T. denticola genomic DNA were probed with a biotinylated internal oppA fragment (opp) and the 2.1-kb ermF/AM cassette (erm). The ermF/AM cassette contains a single ScaI site. (B) Western immunoblot of T. denticola lysates probed with anti-70 antibodies. Lanes: 1, parent strain ATCC 35405; 2, oppA mutant strain OHE; 3, oppF mutant strain OXE.
