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. 2022 Nov 29;23(23):14951. doi: 10.3390/ijms232314951

Table 3.

NO-dependent changes in the expression of genes related to GA metabolism and signalling in seeds.

Gene Changes in Gene
Expression
Plant Material General Description of the Material
and Results
References
Biosynthesis GA3ox2 Hedge mustard
seeds
Downregulated at 3, 6, 22, and 26 h of imbibition of non-after-ripened seeds in 20 mM KNO3. [80]
GA20ox2 ↑↓ Upregulated only at 3 h and downregulated after 6, 12, and 26 h of imbibition of non-after-ripened seeds in 20 mM KNO3.
GA2ox6 Downregulated after 22 and 26 h of imbibition of non-after-ripened seeds in 20 mM KNO3.
GA3ox2 Hedge mustard
seeds
Stimulated by 20 mM KNO3, especially at 22 and 26 h of imbibition of 7-months after-ripened seeds. [81]
GA20ox2 Upregulated between 6 and 26 h of imbibition of 7-month after-ripened seeds treated with 20 mM KNO3.
GA2ox6
GA3ox1 Arabidopsis seeds CN alleviates the dormancy of Arabidopsis seeds. Seed treatment with 200 µM cPTIO reduced gene expression compared with single CN application. [85]
GA3ox2
Signalling RGL2 Hedge mustard
seeds
Downregulated after 22 and 26 h of imbibition of non-after-ripened seeds in 20 mM KNO3. [80]
RGL2 Hedge mustard
seeds
In 7-months after-ripened seeds, expression was negatively affected by 20 mM KNO3 at 6 and 22 h of imbibition. [81]

↑ upregulation, ↓ downregulation.