Table 4.

NO-dependent changes in the expression of genes related to ET metabolism and signalling in seeds.

	Gene	Changes in Gene Expression	Plant Material	General Description of the Material and Results	References
Biosynthesis	ACS7	↑	Hedge mustard seeds	After 20 mM KNO3 treatment, the ACS7 transcript was expressed only at the beginning of imbibition (3 h) in seeds that were not after-ripened, and after-ripening eliminated this expression.	[86]
	ACO2	↑/↓		After 20 mM KNO3 treatment, the transcript level was very high in non-after-ripened seeds at 3 h of imbibition and strongly diminished up to 12 h, increasing afterwards; after-ripening reduced transcript accumulation during the first 6 h of imbibition.
	ACS1	↑	Wild cabbage seeds	Upregulated between 12 and 48 h of imbibition in seeds treated with 5 mM NO.	[72]
	ACS3
	ACS7
	ACS11
	ACS9			Increased at 12 and 24 h of imbibition of seeds treated with 5 mM NO.
	ACS4	↓		Decreased between 12 and 48 h of imbibition in seeds treated with 5 mM NO.
	ACS5
	ACO1	↓/↑		Downregulated after 12 h and upregulated after 24 and 36 h of imbibition in seeds treated with 5 mM NO.
Signalling	ETR1	↑	Wild cabbage seeds	Upregulated between 12 and 48 h of imbibition in seeds treated with 5 mM NO.	[72]
	ETR2

↑ upregulation, ↓ downregulation.