Figure 5.

Effects of SIP on melanogenesis-related protein expression levels in FSK-stimulated B16F10 melanoma cells. (A) Protein expression levels of TYR, TRP-1, and TRP-2 were determined by Western blot following SIP treatment for 72 h with the indicated concentration. The densitometric analysis of TYR (B), TRP-1 (C), and TRP-2 (D) using Photoshop software2020 (Adobe, San Jose, CA, USA). Results were presented as mean ± SD. Statistical analysis was performed with one-way ANOVA, followed by Tukey’s multiple comparison test (n = 3; * p < 0.05, *** p < 0.001, and **** p < 0.0001 compared with the forskolin-stimulated group).