TABLE 2.
LPS prepn | EU/ng of LPS | pg of TNF-α/ng of LPSa | pg of TNF-α/EU | LD50b |
---|---|---|---|---|
E. coli 2 | 13 | 2.9 × 104 | 2.2 × 103 | 12 |
S. marcescens 1 | 14 | 1.0 × 104 | 7.5 × 102 | 17 |
C. diversus 2 | 15 | 1.5 × 104 | 1.0 × 103 | |
C. diversus 1 | 16 | 1.5 × 104 | 9.2 × 102 | 10 |
P. mirabilis 2 | 24 | 2.0 × 104 | 8.0 × 102 | 15 |
Values were calculated by dividing the amount of TNF-α produced in the supernatant (pg/ml) by the dose (ng/ml) of LPS used to stimulate J774A.1 cells. These values were taken from the linear portion of the dose-response curve for the individual LPS preparation.
LD50 values were determined by sensitizing mice with d-galactosamine as described in Materials and Methods. For each LPS, two control mice (no LPS) were included. Lethality was determined by using doses of 5, 10, 15, and 20 ng of LPS per 25-g mouse. Five mice were used at each dose. Thus, including controls, a total of 22 mice were used for each LPS preparation.