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. 2022 Nov 26;14(23):5149. doi: 10.3390/polym14235149

Figure 7.

Figure 7

Microscopic examination of the ovine contralateral carotid artery. Centre: haematoxylin and eosin staining, van Gieson staining, alizarin red S staining, and EM-BSEM analysis, ×100 magnification, scale bar: 100 µm (for EM-BSEM analysis, scale bar: 500 µm). Left top: immunofluorescence staining for ECs (CD31 or vWF) and VSMCs (α-SMA), ×400 magnification, scale bar: 50 µm. Right top: immunofluorescence staining for medial collagens (type III and type IV), ×200 magnification, scale bar: 100 µm. Nuclei are counterstained with DAPI. Left bottom: EM-BSEM analysis, lamellar units and tunica adventitia, ×250 magnification, scale bar: 200 µm. Right bottom: EM-BSEM analysis, ECs and VSMCs, ×1000 magnification, scale bar: 50 µm. Note the EC monolayer, regular lamellar units (elastic laminae and VSMCs between them), and collagen-rich tunica adventitia.