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. 2022 Dec 2;23(23):15180. doi: 10.3390/ijms232315180

Figure 3.

Figure 3

Induction of NETs and its association with BC. (A) The mRNA expression of HIST3H3 in different cancers and adjacent normal tissues was analyzed by TIMER. Red box indicated the breast cancer and the adjacent normal tissues. (B) The correlation between HIST3H3 expression and neutrophil infiltration in BC was analyzed by TIMER. (C) The correlation between copy number alteration of HIST3H3 and neutrophil infiltration was analyzed by TIMER. (D) PMNs were extracted from the femur bone of ICR mice and cultured for 2 h, 4 h, and 8 h. Thereafter, the cell viability was determined by MTT assay. Data were represented as mean ± SD, n = 3. * p < 0.05 and ** p < 0.01 compared with the group of 0 h. (E) PMNs were treated with PMA (15–60 nM) for 6 h. The cell morphology was determined by Hoechst 33342 staining. (F) PMNs were treated with PMA (15–60 nM) for 6 h. The H3cit expression was determined by Western Blot. Data were represented as mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the control group.