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Affinity chromatography for SecA2. (A,B) Identification of SecA2 in cytosolic fractions BC (before column), AC (after column) and elution fractions (E1–E6) using specific anti-SecA2 antibody; and (C) comparison of affinity chromatography purified Strep-tagged SecA1 (96 kDa) and SecA2 (90 kDa). M = Size ladder.
