Figure 5.

(A) Differences in the amounts of secreted RNA (sec-RNA) isolated from the supernatant of wild type Lm, Lm-∆secA2 and Lm-∆secA2::SecA2_strep; (B) correlation between SecA2 protein concentration and its co-eluted RNA. Protein and RNA concentration of cytosolic fractions before (BC), after (AC) passing through the streptactin chromatography column, in wash steps (W1-W8) and elution fractions (E1-E10); and (C) the corresponding coomassie gel for SecA2 protein. Data are presented as means ± SD of results from three experiments * p ≤ 0.05; ** p ≤ 0.01.