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(A) HPLC chromatogram recorded at 330 nm of the standards and the reactions products of NBD-O-CmCH2OH (60 µM) with various biothiols (each 300 µM). (B) HPLC chromatogram recorded at 530 nm of the NBD-SH standard and the reaction mixtures of probe and NBC-Cl (60 µM) with Na2S (300 µM). The incubation time was 15 min.
