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(A) HPLC chromatogram of the standards (60 µM) and reaction mixtures of NBD-O-CmCH2OH (60 µM) with Na2S (0–300 µM) after 15 min incubation. The traces were collected using an absorption detector set at 330 nm. (B) HPLC-based titration of NBD-O-CmCH2OH (60 µM) with Na2S after 15 min of incubation in solution. Data are means ± standard deviation of three independent experiments.
