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Characteristic absorption bands and retention times of thiol-specific products formed in the reaction of NBD-O-CmCH2OH with H2S, GSH, L-Cys, and NAC.
| Thiol-Specific Product | λmax (nm) | Retention Time (min) |
|---|---|---|
| NBD-SH | 541 | 2.99 |
| NBD-GSH | 418 | 2.50 |
| NBD-Cys | 475 | 3.45 |
| NBD-NAC | 543, 424 | 3.25 |
