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. 2022 Nov 29;11(23):3828. doi: 10.3390/cells11233828

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Exosomal and melanocytic markers present in UM-derived EVs. Cell pellets and EV fractions isolated from UM cell lines TJU-UM001 (metastatic), Mµ2 (non-metastatic), and Mµ2F (metastatic), or NCMs, were analyzed for their expression of exosomal (CD9, CD63, CD81, Tsg101) or melanocytic (Melan-A, TYRP1) markers by Western blotting. The anti-α-tubulin was used as marker of cytosolic proteins. Ponceau S staining was used as a loading control. Arrows indicate the specific band for each marker.