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. 2022 Nov 29;11(23):3828. doi: 10.3390/cells11233828

Figure A1.

Figure A1

Dynasore and proteinase K pretreatments prevented EV internalization in stellate cells. (A) Fluorescence of HSteCs without addition of EVs by flow cytometry. Fluorescence of HSteCs after the addition of DiI-labeled large EVs derived from UM cell lines (Mµ2F, TJU-UM001) or NCMs for 24 h (A,B). (B) Fluorescence of HSteCs pretreated with Dynasore or after the addition of TJU-UM001-EVs pretreated with proteinase K are shown as controls. The gates indicate the percentage of fluorescent HSteCs.