Figure A2.

Dynasore and proteinase K pretreatments prevented UM-derived EV internalization in endothelial cells and inhibit the formation of capillary-like tubes. Untreated GFP-HUVECs were cultured for 12 h on Matrigel^® with medium without angiogenic factors (negative control; black bars). Large EVs isolated from metastatic UM cell line TJU-UM001 were added to GFP-HUVECs for 24 h before their seeding on Matrigel^® for 12 h (white bars). GFP-HUVECs were also pretreated with Dynasore (dark grey bars), or TJU-UM001-EVs were pretreated with proteinase K (light grey bars) as controls. The number of junctions, master junctions, nodes, and meshes were quantified in function of time (hours) for each condition (n = 1).