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. 2022 Dec 2;23(23):15183. doi: 10.3390/ijms232315183

Table 3.

Ranking the assessment of the biological test results.

Criterion Parameter
Unacceptable Level
(Score = 0)
Moderate Level
(Score = 1)
Optimal Level
(Score = 2)
Direct cytotoxicity, % (relative cell viability in the MTT test) Bare NiTi control’s level
or less (≤72%), high surface cytotoxicity
> Bare NiTi control ≤85%
(73–85%),
moderate cytotoxicity
>85%,
low cytotoxicity
Cell adhesion, %
(390 cells/mm2 = 100% adhesion on the bare NiTi surface)
<150% of the bare NiTi control’s level,
low adhesion
150–250% of the bare NiTi control’s level,
moderate adhesion
>250% of the bare NiTi control’s level,
high adhesion
Cell viability, % (relative content of living cells on the sample’s surface) <85%,
non-cytocompatible surface
- ≥85%,
cytocompatible surface
NO production, μM/mL,
(16 μM/mL- NO production on the bare NiTi surface)
Bare NiTi control’s level
or less
(<16 μM/mL),
low functional activity
>Bare NiTi control, but <150% of the bare NiTi control’s level
(16–24 μM/mL),
moderate functional activity
>150% of the bare NiTi control’s level (>24 μM/mL).
high functional activity