TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant featuresa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| RK5048 | metE70 tonB | 26 |
| H1717 | aroB fhuF::λ placMu; Kmr | 58 |
| XL1-Blue | High efficiency transformation; Tcr | Stratagene |
| SM10 | Mobilizing strain; Kmr | 57 |
| BL21(DE3)/pLysS | High-stringency expression host; Cmr | Novagen |
| B. avium 103004 | Smr | Institut Pasteur, Paris, France |
| B. bronchiseptica BB1015 | Smr but not rpsL | 51 |
| B. parapertussis PEP | Smr | 45 |
| B. pertussis | ||
| BPSM | Derivative of Tohama I rpsL; Smr Nalr | 41 |
| BPEP98 | Derivative of BPSM but ΔtonB exbB::ΩKmr | This study |
| BPLOW | Derivative of BPSM but ΔbvgAS | E. Willery, Lille, France |
| BPEP184 | Derivative of BPSM but alcR::Kmr | 51 |
| BPEP269 | Derivative of BPSM with pEP636 integration | This study |
| BPEP270 | Derivative of BPEP98 with pEP636 integration | This study |
| Plasmids | ||
| pSUTonBExbBD | pSU19 containing E. coli tonB, exbB, and exbD; Cmr | R. Kadner |
| pCG475 | pUC18 bearing BPSM tonB exbB exbD basR on a 3.1-kb EcoRI fragment; Apr | This study |
| pEP487 | pCG475 with a BamHI ΩKmr cassette inserted in the exbB BclI site; Apr Kmr | This study |
| pHP45Ω-Km | Source of ΩKmr cassette | 20 |
| pBCSK+ | High-copy-number vector; Cmr | Stratagene |
| pEP498 | pBSCK+ bearing BPSM tonB exbB exbD basR′ on a 3-kb EcoRI-XhoI fragment; Cmr | This study |
| pEP532 | pBSCK+ bearing the BB1015 tonB upstream region, (′metYpiuChupB) on a 2.4-kb EcoRI fragment; Cmr | This study |
| pJQ200mp18rpsL | Bordetella suicide vector, contains E. coli rpsL; Gnr | D. Raze, Little, France |
| pEP491 | pJQ200mp18rpsL bearing tonB exbB::ΩKmrexbB basR on an EcoRI fragment isolated from pEP487; Gnr Kmr | This study |
| pEP549 | Derivative of pEP491 through SalI deletion, bears ΩKmrexbD basR; Gnr Kmr | This study |
| pEP552 | Derivative of pEP549 through insertion of a 1.3-kb DNA region 5′ of tonB on the chromosome; Gnr Kmr | This study |
| pET24a+ | T7 promoter expression vector; Kmr | Novagen |
| pEP583 | pET24a+ derivative to produce Bp TonB; Kmr | This study |
| pJQ200mp18 | Bordetella suicide vctor; Gnr | 52 |
| pEP636 | pJQ200mp18 bearing ′piuC hupB tonB exbBD basR′ on a 4.5-kb XbaI-XhoI fragment; Gnr | This study |
a
Apr, Cmr, Gnr, Kmr, Nalr, Smr, and Tcr, resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, streptomycin and tetracyclin, respectively.