FIGURE 5.

Nsun5 affected oocyte function by inhibiting the m⁵C modification of Mad2l2. (B and C) Relative luciferase activity of Hoechst (blue), NSUN5 (red) and MAD2L2 (green) in the Nsun5 ^KO and WT groups (*p < .05, **p < .01). (A) Protein expression of MAD2L2 in the Nsun5 ^KO and WT groups (*p < .05). (D) Co‐immunoprecipitation (Co‐IP) showing the interaction between NSUN5 and MAD2L2. (E) Representative m⁵C sites of Mad2l2 on chromosome 4 in the WT group. (F) Representative m⁵C sites of Mad2l2 on chromosome 4 at 6 months in the Nsun5^KO and WT groups. (G) Differential methylation rates of two common m⁵C sites at 6 months (*p < .05). (H) MAD2L2 mRNA stability of NSUN5 ^KD and control KGN cells (***p < .001). (I) mRNA stability of MAD2L2 in the control, NSUN5 ^KD, site 1^Mut and site 3^Mut groups (***p < .001). (J) Luciferase expression of MAD2L2‐WT, MAD2L2‐site 1^Mut and MAD2L2‐site 3^Mut in the control and NSUN5 ^KD cell lines (***p < .001)