TABLE 1.
Bacteria and plasmids used in the study
| Strain | Serogroup and/or description | Source and/or reference |
|---|---|---|
| V. choleraea | ||
| CO604 | O10 (4) | This work (clinical isolate) |
| CO848b | O26 Strs (32) | This work (clinical isolate) |
| AS16 | O144 (8) | This work (clinical isolate) |
| AS233 | O151 (16) | This work (clinical isolate) |
| DO40 | O37 (4) | This work (clinical isolate) |
| DO42 | O19 (4) | This work (clinical isolate) |
| DO45 | O8 (8) | This work (clinical isolate) |
| DO50 | O107 (16) | This work (clinical isolate) |
| DO56 | O145 (16) | This work (clinical isolate) |
| DO57 | O2 (8) | This work (clinical isolate) |
| DO62 | O186 (16) | This work (clinical isolate) |
| DO61 | O145 (4) | This work (clinical isolate) |
| DO69 | O145 (2) | This work (clinical isolate) |
| DO71 | O37 (2) | This work (clinical isolate) |
| DO76 | O125 (16) | This work (clinical isolate) |
| DO77 | O37 (16) | This work (clinical isolate) |
| CLO5 | O169 (16) | This work (clinical isolate) |
| CO853 | O139 (8) | This work (clinical isolate) |
| CO848b-str | O26 (32) | Spontaneous Strr mutant of CO848b |
| E. coli | ||
| SM10λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Kmr (λpir) | 16 |
| S17-1λpir | Tpr SmrrecA thi pro hsdR−M+ RP4:2-Tc:Mu:Kmr Tn7 (λpir) | 7 |
| Plasmids | ||
| pBluescript | Ampr | 24 |
| pBR322 | Tetr | 4 |
| pKAS46 | Ampr Kmr | 25 |
| pRM | pBluescript hlyA | This work |
| pRM1 | pBluescript hlyA::tet | This work |
| pRM2 | pKAS46 hlyA::tet | This work |
a
All V. cholerae strains lacked ctxAB, zot, and ace but had the hlyA gene. Also, 92 other strains of various serogroups had no vacuolating titer. The value in parentheses is the reciprocal of the cell vacuolating activity of nonconcentrated culture supernatants.