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. 2022 Dec 6;23(23):15374. doi: 10.3390/ijms232315374

Figure 1.

Figure 1

ERβ expression in melanoma cells of male and female patients. mRNA (A) and protein (B) levels of ERβ in female (WM266-4, M21) and male melanoma cells (HS294t, SKMEL2) grown in standard (pH 7.4) or acidic medium (pH 6.7) for 24 h. mRNA level and protein expression of ERβ in M51 (female) and SSM2c (male) melanoma cells grown in an acidic medium for 24 h, performed by Real-time PCR (C), Western blotting (D), and flow cytometry (E). Histograms represent the mean ± SEM of at least three independent experiments. * p < 0.05 compared with control cells.