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. 2022 Nov 22;14(23):5728. doi: 10.3390/cancers14235728

Figure 3.

Figure 3

MK5 regulates cellular motility through post-translational modification of focal adhesion proteins and HSP27, as well as regulation of ERK3 stability in LNCaP and PC3 cells. (A) Immunoblots of the indicated proteins in LNCaP and PC3 cells treated with different concentrations of GLPG0259. GAPDH was used as a loading control. Images are the representation of three independent experiments (n = 3). (B–E) Quantifications of (B) Phospho-FAK Y861 level normalized to total FAK; (C) Phospho-Paxillin Y118 level normalized to total paxillin; (D) Phospho-HSP27 S82 normalized to total HSP27; and (E) ERK3 level normalized to GAPDH in LNCaP and PC3 cells. VC = vehicle control (DMSO). One-way ANOVA, followed by Tukey’s post hoc analysis, was used for multiple group comparison. Groups which are significantly different from each other are marked by asterisks: * = p < 0.05, ** = p < 0.005, and *** = p < 0.0005. Error bar represents standard error of the mean (SEM). The whole blots of Figure 3 could be found in Figure S2.