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. 2022 Nov 22;14(23):5728. doi: 10.3390/cancers14235728

Figure 5.

Figure 5

MK5 regulates matrix metalloproteinases’ expression in PC3 cells. (A–C) mRNA expression of several matrix metalloproteinases (MMPs): (A) MMP2, (B) MMP9, and (C) MMP10 in LNCaP and PC3 cells treated with different concentrations of GLPG. GAPDH mRNA was used as an internal control. (D) Immunoblots of the indicated proteins in LNCaP and PC3 cells treated with different concentrations of GLPG. GAPDH was used as a loading control. Images are the representation of three independent experiments (n = 3). (E,F) Quantifications of (E) MMP2, and (F) MMP9 level normalized to GAPDH in LNCaP and PC3 cells. (G) Immunoblots of the indicated proteins in PC3 cells upon MK5 depletion. GAPDH was used as a loading control. (H–J) Quantifications of (H) ERK3, (I) MMP2, and (J) MMP9 level normalized to GAPDH in PC3 cells upon MK5 depletion. VC = vehicle control (DMSO); shSCR = scrambled shRNA. One-way ANOVA, followed by Tukey’s post hoc analysis, was used for multiple group comparison: * = p < 0.05, ** = p < 0.005, and *** = p < 0.0005. Error bar represents standard error of the mean (SEM). The whole blots of Figure 5 could be found in Figure S2.