Figure 4.

Baicalin inhibits A375 metastasis. (A) BALB/c-Nude mice were i.v. engrafted with 5 × 10⁶ A375 cells (n = 8, each group). (B) Metastatic colonies formed in the liver, spleen, kidney, and lymph node 60 days after A375 melanoma cells injection, with 50 mg/kg, 100 mg/kg Baicalin or saline. (C) Metastasis rate of mouse injected A375 cells treated with or without Baicalin. (D) BALB/c-Nude mice were i.v. engrafted with 5 × 10⁶ A375M cells (n = 8, each group). (E) Metastatic colonies formed in the liver at 21 days and mice were sacrificed at day 35 after A375M melanoma cell injection, with 50 mg/kg, 100 mg/kg Baicalin or saline treatment. HE staining patterns showed blue. 40×. Scale bar, 200 μm. (F) The number of metastatic nodes was counted. (G,H) Measurement of A375 cell invasion ability using 3D stroma invasion assay with or without incubating with Baicalin for 24 h. (I,J) Measurement of A375 cell migration ability using the trans-well system with or without incubating with Baicalin for 24 h. Views were selected randomly from each sample and migrated cells were counted (three views were randomly selected for each group). 100×. Scale bar, 200 μm. (K,L) Measurement of A375 and A375 cell migration ability using a wound-healing assay with or without incubating with Baicalin for 24 h. Views were selected randomly from each sample. 100×. Scale bar, 200 μm. Data are expressed as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.