Table 2.
Sequences of primers used for RT-PCR.
| Gene | Primer Sequence (5′ to 3′) | Product Size | Accession | |
|---|---|---|---|---|
| Cd36 | F: GTGCAAAACCCAGATGACGT | R: TCCAACAGACAGTGAAGGCT | 180 | NM_001159558.1 |
| Chrebp | F: GTGTGTGGTTTCGTGACCC | R: CACTTGTGGTATTCGCGCATC | 128 | NM_001359237.1 |
| Srebp-1c | F: ATCGCAAACAAGCTGACCTG | R: AGATCCAGGTTTGAGGTGGG | 115 | NM_001388389.1 |
| Fasn | F: TCCTGGAACGAGAACACGATCT | R: GAGACGTGTCACTCCTGGACTTG | 138 | NM_007988.3 |
| Cpt1β | F: GGCACCTCTTCTGCCTTTAC | R: TTTGGGTCAAACATGCAGAT | 136 | NM_009948.2 |
| Clock | F: ATGGTGTTTACCGTAAGCTGTAG | R: CTCGCGTTACCAGGAAGCAT | 197 | XM_011249402.3 |
| Bmal1 | F: CAGAGCCGGAGCAGGAAAAATAGGT | R: CAGGGGGAGGCGTACTTGTGATGT | 128 | NM_001374642.1 |
| Per1 | F: CGGATTGTCTATATTTCGGAGCA | R: TGGGCAGTCGAGATGGTGTA | 142 | NM_001159367.2 |
| Per2 | F: GAAAGCTGTCACCACCATAGAA | R: AACTCGCACTTCCTTTTCAGG | 186 | NM_011066.3 |
| Cry1 | F: CACTGGTTCCGAAAGGGACTC | R: CTGAAGCAAAAATCGCCACCT | 153 | NM_007771.3 |
| Cry2 | F: CACTGGTTCCGCAAAGGACTA | R: CCACGGGTCGAGGATGTAGA | 102 | NM_009963.4 |
| Rorα | F: TCCAAATCCCACCTGGAAAC | R: GGAAGGTCTGCCACGTTATCTG | 70 | NM_001289916.1 |
| Rve-erbα | F: TACATTGGCTCTAGTGGCTCC | R: CAGTAGGTGATGGTGGGAAGTA | 127 | NM_145434.4 |
| Rve-erbβ | F: GGAAACACTCATCCGTGCACTA | R: ATCGAAGATCTGGCAACTTTAGAA | 101 | NM_001145425.2 |
| Ucp1 | F: TAAGCCGGCTGAGATCTTGT | R: GGCCTCTACGACTCAGTCCA | 84 | NM_009463.3 |
| Ucp3 | F: CTGCACCGCCAGATGAGTTT | R: ATCATGGCTTGAAATCGGACC | 191 | NM_009464.3 |
| Pgc-1α | F: TATGGAGTGACATAGAGTGTGCT | R: CCACTTCAATCCACCCAGAAAG | 134 | NM_008904.3 |
| Hsp70 | F: CGGTGCCCGCCTACTTC | R: TCCTTCTTGTGCTTCCTCTTGA | 322 | NM_005346.6 |
| Hsp90 | F: ACGAGGAAGAGAAGAAGAAAATGG | R: GCAGGGTGAAGACACAAGCC | 131 | NM_001271971.2 |
| P23 | F: ATGCGTTTGGAGAAGGACAGA | R: CAGGGATGAAGTGATGGTGAG | 210 | NM_001289785.1 |
| Gapdh | F: CCGAGAATGGGAAGCTTGTC | R: TTCTCGTGGTTCACACCCATC | 232 | NM_001289726.1 |
F = forward primer; R = reverse primer.