Figure 3.

The dose-dependent effect of pharmacological inhibition of lipid handling proteins on the uptake of exogenously added free FAs in A2780 (A,B) or SKOV3 (C,D) OC cells. Cells were treated for 48 h with increasing doses of the small molecular inhibitors BMS309403, HTS01037, NAV2729, SB-FI-26, or SSO before FA uptake was ascertained by the QBT™ Fatty Acid Uptake Kit as specified in the Materials and Methods Section (A,C). Note that the concentrations of SSO are plotted on a logarithmic scale at the top of the graphs (A,C), while the concentrations of all other inhibitors are plotted on a linear scale at the bottom. Obtained dose–response curves were used to determine the drug-specific IC₅₀ values and to find optimal drug concentrations for subsequent time course treatments (B,D). Fluorescent signals were normalized to the optical density values that were concurrently determined by formazan dye cell proliferation assays as an indirect readout for cell numbers and to the direct cell counts determined visually in a hemocytometer. Normalized data were expressed in % of solvent (0.1% DMSO)-treated controls. Means ± SD of three separate assays with replicates. ANOVA followed by Scheffe test, p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) relative to solvent-treated cells.