Figure 4.

The time-dependent effect of pharmacological inhibition of lipid handling proteins on the uptake of exogenously supplied FAs in A2780 and SKOV3 OC cells. Drug concentrations close to the IC₅₀ values for inhibition of cell growth and uptake of exogenous FA (A2780: 10 µM NAV2729, 30 µM BMS309403, 30 µM HTS01037, 30 µM SB-FI-26, and 100 µM SSO; SKOV3: 10 µM NAV2729, 80 µM BMS309403, 80 µM HTS01037, 80 µM SB-FI-26, and 200 µM SSO) were applied for time course experiments. Fluorescence signals were normalized to cell numbers that were concurrently determined by formazan dye cell proliferation assays and hemocytometer counting, and were expressed in % of solvent(0.1% DMSO)-treated control cells. Means ± SD of three separate assays with replicates. ANOVA followed by Scheffe test, p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) relative to solvent-treated cells.