Figure 5.

The time-dependent effect of pharmacological inhibition of lipid handling proteins on the uptake of exogenously supplied LDLs in A2780 and SKOV3 OC cells. Cells were exposed for various times to the same doses of the small molecule inhibitors BMS309403, HTS01037, NAV2729, SB-FI-26, or SSO as in Figure 4 before LDL uptake was measured with the pHrodo™ Red LDL Uptake Kit according to the procedure given in the Materials and Methods Section. Fluorescent signals were normalized to the optical density values that were concurrently determined by formazan dye cell proliferation assays as an indirect readout for cell numbers and to the direct cell counts determined visually in a hemocytometer. Normalized data were expressed in % of solvent (0.1% DMSO)-treated controls. Means ± SD of three separate assays with replicates. ANOVA followed by Scheffe test, p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) relative to solvent-treated cells.