Figure 7.

The effect of pharmacological inhibition of lipid handling proteins on apoptosis of OC cells. A2780 cells were incubated in medium containing solvent (negative control), 7 µM of the multi-kinase inhibitor ponatinib (positive control) [39], or various concentrations of small molecule inhibitors of cellular lipid handling proteins at 37 °C for 48 h. Then, cells were examined by flow cytometry to determine the percentage of early apoptotic, APC annexin V-positive cells (A) or later apoptotic, active caspase-3-positive cells (B). Results show relative cell numbers in percent of total and represent the means ± SD of three independent experiments. The level of significance was determined by ANOVA followed by Scheffe test, p < 0.01 (**), and p < 0.001 (***) relative to solvent (negative control).