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. 2022 Nov 22;11(23):3712. doi: 10.3390/cells11233712

Figure 1.

Figure 1

Single-cell experiments comparing efferocytic BM macrophages (engulfing apoptotic cancer cells) vs. control (non-engulfing BM macrophages). (A) BM macrophages (MΦ) were isolated from 4 wk old C57BL/6J mice and co-cultured with apoptotic RM1(a) cells (CFSE labeled) for 16–18 h. Efferocytic (F4/80+CFSE+) and control BM macrophages (F4/80+) were sorted and used for single-cell libraries followed by scRNA-Seq. (B) UMAP of all cells analyzed (blue: efferocytic BM macrophages; red: non-efferocytic BM macrophages). (C) Heatmap of the most differentially expressed genes in either experiment. (D) Venn diagram of all differentially expressed genes and their overlap between both experiments. (E) Cleveland plot of the most enriched pathways in efferocytic BM macrophages. (F) Dot plots of hypoxia-related genes in both experiments. Size relates to the percentage of macrophage cells expressed each gene. Color denotes the average expression for each gene across all expressing cells. Additional results are shown in Appendix A—Table A1, Table A2 and Table A3.