Figure 6.

Efferocytic BM macrophages stabilize HIF-1α and induce MIF expression. BM macrophages (MΦ) were isolated from C57BL/6J mice and co-cultured alone or with apoptotic RM1(a) cells and treated with FG-4592 (Roxadustat, a HIF prolyl-hydroxylase inhibitor (10 µM)) or vehicle control for 16–18 h. (A) Protein lysates from co-cultures were analyzed by Western blotting with HIF-1α and MIF antibodies (n = 12/group). (B) mRNAs were isolated from co-cultures and analyzed by RT-qPCR for Mif expression (n = 9/group). Graphs show the fold change of each group relative to vehicle-treated BM macrophage control. The Western blots shown in A are representative images. Plotted data are shown as mean ± SEM; * p < 0.05, ** p < 0.01, **** p < 0.0001, ns = not significant (repeated measures one-way ANOVA; Tukey’s multiple comparison test).