FIG. 1.

CHO hSR-AII cells associate with more E. coli K-12 than CHO WT cells. (A) CHO hSR-AII and CHO WT cells were incubated with heat-killed FITC-labelled E. coli K-12 (200 bacteria per cell) for 45 min at 37°C and analyzed by flow cytometry. The dotted line denotes control cells incubated with medium alone, and the solid line shows the increase in fluorescence after incubation with fluoresceinated bacteria. (B) CHO hSR-AII and CHO WT cells were incubated with increasing numbers of FITC-labelled E. coli K-12 organisms per cell for 45 min and analyzed as above. The mean fluorescence for each assay condition was averaged with the corresponding duplicate and plotted along with the standard deviation calculated for each assay condition. (C) FITC-labelled E. coli K-12 (200 bacteria per CHO cell) was incubated with transfected or control CHO cells for various times at 37°C and analyzed as above.