TABLE 2.

Effect of culture conditions on E. coli uptake by different cell populations^a via SR-A-dependent and -independent mechanisms

Culture condition	% Uptake of E. coli by:
	BMMφb		MDMc		CHOd
	SR-A	Non-SR-A	SR	Non-SR	WT	hSR-AI	hSR-AII
Adhesion to TCP	0	100	60	40	10	40	100
Adhesion to BPe	55	45	70	30

^aCells were cultivated on the appropriate substratum in the presence of FCS. The results shown are from single assays but are representative of at least three similar experiments. 

^bPercentages are derived from comparison of the levels of E. coli ingestion by 129/ICR and SR-A^−/− Mφ. 

^cPercentages are derived from comparison of the level of E. coli uptake in the presence or absence of the general SR inhibitor, poly(I). 

^dPercentages were derived from comparison of the level of E. coli uptake by CHO hSR-AII. 

^eBMMφ plated on BP and activated by LPS had no effect on SR-A-mediated uptake but increased SR-A-independent ingestion of E. coli.