Figure 5.

Quantitative phase imaging (QPI) and SHSY-5Y cell proliferation analysis. (a) Representative images of cells pretreated for 24 h with natural astaxanthin (NAst), synthetic astaxanthin (ast), and vehicle (ctrl), acquired at 0 h and after 72 h of incubation with RSL3. (b) Plot of cell count over time. Coloured lines are for control cells (blue line) and for cells treated with synthetic astaxanthin (yellow line), synthetic astaxanthin and RSL3 (pink line), NAst (green line), NAst and RSL3 (brown line), and RSL3 alone (purple line), for up to 72 h. Each time point represents the average of five independent wells ± SEMs. Scale bar: 200 µm.