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. 2022 Nov 28;11(23):7044. doi: 10.3390/jcm11237044

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative TEM-images visualize the pellicle ultrastructure. A subsurface pellicle can be detected after rinsing with fragaria vesca extract (a) and stannous fluoride (b) after 8 h of oral exposition [119,147]. Demineralized areas are filled with proteinaceous structures (arrows) and stannous ions (b). A higher electron density of the pellicle’s basal layer can be observed after rinsing with stannous ion containing agents. The enamel was removed during specimen processing and the former enamel side is marked by an asterisk.