Figure 4.

Monitoring of the intracellular calcium cycling properties on 14-day-old neuro–cardiac OOC using PC12 and hiPSC-CMs. (A) Schematic representation of neurocardiac OOCs with cellular compartments showing where the different molecules are added. The M2 muscarinic receptor antagonist Atropine is added to the cardiac compartment and the muscarinic receptor agonist carbachol is added to the neuronal compartment. (B) CaT are recorded using Fluo4-AM. Representation of CaT in hiPSC-CMs recorded in line-scan configuration with the corresponding plot below from which calcium kinetics are obtained. (C) Parameters of normalized amplitude of CaT, (D) calcium release velocity, (E) duration of Ca²⁺ reuptake and (F) frequency of CaT are presented in resting condition, after addition of 1.5 mM Carbachol and or 1 µM Atropine on the cardiomyocytes located in the cardiac compartment after 14 days of co-culture. Data are presented as mean ± SEM, Rest n = 145, Carbachol n = 108, Atropine n = 34, Carbachol + Atropine n = 55. Dunn’s multiple comparisons test were performed, * p < 0.05, ** p < 0.01, *** p < 0.001.