Figure 7.

Activation of SIRT1/Nrf2 signaling attenuates the antioxidant effect of elaiophylin in A549 cells. The cells were divided into six groups: control, SIRT1 overexpression only, Nrf2 overexpression only, elaiophylin, elaiophylin+SIRT1 activation (SIRT1 overexpression plus SRT1720), elaiophylin+Nrf2 activation (Nrf2 overexpression plus DMF). (A,B) Intracellular ROS level was assessed using DCFH-DA fluorescence probe, and mitochondrial ROS level was evaluated using Mitosox Red fluorescence probe. (C) Mitochondrial membrane potential was assessed by rhodamine 123 staining. Data were expressed as mean ± SD of three experiments. ** p < 0.01 vs. control, ^## p < 0.01 vs. elaiophylin group.