Figure 8.

Elaiophylin suppresses tumor growth in an A549-xenograft model by inhibiting SIRT1. (A) After the elaiophylin treatment, the subcutaneous graft tumor was stripped and measured (n = 5). The image of tumor samples was shown on the left, and the volume change measured was shown on the right. (B) The weight changes in subcutaneously transplanted tumor mice with or without elaiophylin treatment were recorded. (C) In vivo fluorescence imaging of luc-A549 subcutaneous grafted tumors after fluorescein potassium injection. (D) The statistical analysis of fluorescence intensity in (C). (E) H&E staining of tumor sections of Luc-A549-xenograft mice treated with or without elaiophylin (the left panel). TUNEL assay (left middle panel) and immunohistochemistry staining of Ki67 (right middle panel) and SIRT1 staining (right panel) were conducted on tumor sections of Luc-A549-xenograft mice treated with or without elaiophylin. Data were expressed as means ± SD of three experiments and each experiment included triplicated repeats. ** p < 0.01 vs. control.