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. 2022 Nov 28;27(23):8279. doi: 10.3390/molecules27238279

Table 1.

Analysis of ligand-receptor interactions of the docked compounds towards two proteins VEGFR2 (PDB = 3U6J), and Topoisomerase IIα (PDB = 1ZXM) using AutoDock Vina *.

Tested Targets VEGFR2
(PDB = 3U6J) a
Topoisomerase IIα
(PDB = 1ZXM) b
Compounds
Co-crystallized ligand (key interactions) 3 HB with Asp 1064, Cys 919 and Lys 868 1 HB with Ser 149
4a - 1 HB with Ser 149
4b 1HB with Lys 868 -
4c 1HB with Asp 1046 -
4d 3 HB with Tyr 916, Lys 868, and Asp 1046 1 HB with Ser 149
4e 1HB with Cys 919 -
4f Arene-cation with Lys 868 1 HB with Asn 150
4g 3HB with Tyr 916, Lys 868 and Asp 1046 2 HB with Ser 149 and Asn 91
4h 2 HB with Lys 868 and Cys 916 -
4i 2HB with Lys 868 and Cys 919 1 HB with Ser 149
4j 2HB with Thr 916 and Lys 868 1 HB with Ser 149 and Arene-cation with Arg 98
4k 1 HB with Asp 1046 and Arene-cation with Lys 868 3HB with Ser 149, Thr 215 and Asn 120
4l 1HB with Asp 1046 2 HB with Ser 149 and Asn 150
5c 2HB with Cys 919 and Lys 868 -
5e - 2 HB with Asn 150 and Lys 168
5i 2HB with Thr 916 and Cys 919 1 HB with Ala 167
6a 2HB with Thr 916 and Asp 1046 1 HB with Ser 149
6b 1 HB with Cys 919 -
6c 1 HB with Cys 919 2 HB with Arg 98 and Ser 149
7a - -
7b 1HB with Asp 1046 2 HB with Ser 149 and Asn 120
7c - 1 HB with Lys 157 and arene-cation Arg 98
8a Arene-cation Lys 868 arene-cation Arg 98 and Lys 157
8b Arene-cation Lys 868 1 HB with Ser 149
8c 1 HB with Lys 920 -

* Docking calculation using AutoDock Vina software was validated by self-docking step that fulfil RMSD lower than 2. a Binding energy of the docked compounds towards VEGFR2 protein from −9.36 to −24.64 Kcal/mol, and b Binding energy of the docked compounds towards topoisomerase IIα protein from −7.64 to −29.46 Kcal/mol.